Table 1.
Sequences of primers and probes used in this study
| Forward | Reverse | Probe | Amplicon size (bp) | Reference | |
|---|---|---|---|---|---|
| 16 s | GGAGACTGCCAGTGATAAA | GAGGTCGCTTCTCTTTGT | CCTTACGACCAGGGCTACACACGT | 113 | This study |
| kpsM | GTTATATGGGACCGTCATATT | GCCACACTCTTTCGTATC | CCCAGAGCTTGCGGATTATCAATC | 125 | This study |
| chuA | CGATGGGCGAAATGTATAA | GTTAGTTTCCGGACGTAAG | TCTCGATTGGTCGCTTCTATACCA | 98 | This study |
| CTX-M | GAAAGCGAACCGAATCT | GACATCGTCCCATTGAC | ACCTTGTTAACTATAATCCGATTGCGG | 101 | This study |
| sat | AGAAATATGGCATCTGTCACC | CAGACGATATAGTCGGTGTTTC | CAGCAACCATTACTCTGGGACAGC | 97 | This study |
| usp | TGAGTTCTGGTATGAGGAAGA | CCCGTATGAACACCATACAC | TACTGCCTGCTAGTGCTTTCTGCC | 126 | This study |
| soxS | AGGGTTGATTACCAGTATCC | AACATATCGCAACACATCAC | ACAGCGGCAATCAGCGGCGATATA | 70 | This study |
| recA | GAGACAATTTGGTACTCCATC | GATATCCAGTGAAAGCGAAC | CGCCTGGGTGAAGACCGTTCCAT | 90 | This study |
| traT | AGATTGCAGAGCGTACTAAG | ACACGGGTCTGGTATTTATG | CAACGGATAATGTTGCCGCCCTGC | 127 | This study |
| ihfB | GACGGTTGAAGATGCAGTAA | CGGTAGTGCAAAGAGAAACT | AGCATATGGCCTCGACTCTTGCGC | 108 | This study |
| hcaT | GGCATTATGGGAGCAACTAC | CTGGCGAGCAGCAATATAAC | ACCACTATCAACCACGGCAACGCC | 114 | This study |
Primer and probe sets were designed using the PrimerQuest Tool (Integrated DNA Technologies). All probes contained a FAM fluorophore at the 5′ end and a black hole quencher at the 3′ end.