Table 2.

The efficiencies of the primers used in the development of the RT-qPCR study

Primer	Efficiency %
16S (reference gene)	84.3
recA	111.1
CTX-M	99.7
sat	111.6
traT	94.3
chuA	115.6
kpsM	101.7
soxS	103.0
usp	90.2
hcaT	118.9
IhfB	115.3

Primer efficiencies were calculated using serial dilutions of amplicons 1:1000. An efficiency of 80–120% was considered.