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. 2019 Nov 20;10:330. doi: 10.1186/s13287-019-1467-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Both NADH^high and CD133⁺ glioma cells possess the properties of GSCs, but are independent each other. a qRT-PCR analysis showing upregulated stemness-related transcription factor genes Nanog, Oct4, Oligo2, and Sox2 in NADH^high CD133⁺, NADH^high CD133⁻, and NADH^low CD133⁺ subpopulations, compared to NADH^low CD133⁻ in GBM1 and LN229. b, c Limiting dilution assay showed increased sphere formation rate and sphere average diameter of NADH^high/CD133⁺, NADH^high/CD133⁻, and NADH^low/CD133⁺ subpopulations, compared to NADH^low CD133⁻cells in GBM1 and LN229 cell lines. All data are presented as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3 independent experiments)