Fig. 6.

Demonstration of unintended intra-rater variation in brain segmentation. Data is shown prior to affine transformation. (A) Example false color image from a single imaging frame from the OIS system as used for brain segmentation (mouse 2, session 5). (B) Variation in manual segmentation between two sessions by the same reader. Pixels within the first segmentation are colored red while those in the second segmentation are colored green. The overlap between the two segmentations is shown in yellow. (C) Reduced variation (greater overlap) is seen between two segmentations when using guided segmentation.