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. 2019 Oct 3;18(6):6261–6268. doi: 10.3892/ol.2019.10959

Figure 4.

Figure 4.

LINC00707 functions as a molecular miR-145 sponge in A549/DDP cells. (A) Predicted binding sites between LINC00707 and miR-145. (B) Relative expression of miR-145 in A549/DDP cells and parental A549 cells was measured using RT-qPCR. ***P<0.001 vs. A549. (C) miR-145 was upregulated in cells transfected with miR-145 mimics. ***P<0.001 vs. NC. (D) Luciferase activity was measured in cells co-transfected with either WT-LINC00707 or MUT-LINC00707, and miRNA negative control or miR-145 mimics. ***P<0.001 vs. WT-LINC00707. (E) Relative expression levels of miR-145 in A549/DDP cells transfected with either si-LINC00707 or si-NC were measured using RT-qPCR. ***P<0.001 vs. si-NC. LINC00707, long intergenic non-coding RNA 00707; miR, microRNA; RT-qPCR, reverse transcription-quantitative PCR; A549/DDP cells, DDP-resistant A549 cells; si-LINC00707, LINC00707 siRNA; si-NC, siRNA negative control; WT, wild-type; MUT, mutant; NC, negative control.