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. 2019 Oct 3;18(6):6261–6268. doi: 10.3892/ol.2019.10959

Figure 5.

Figure 5.

miR-145 downregulation reverses the effect of LINC00707 knockdown in A549/DDP cells. (A) miR-145 was downregulated in A549/DDP cells transfected with miR-145 inhibitor. (B) Relative expression levels of miR-145 in A549/DDP cells transfected with si-LINC00707 + inhibitor NC or si-LINC00707 + miR-145 inhibitor were measured using RT-qPCR. (C) IC50 values of DDP in A549/DDP cells transfected with either si-LINC00707 + inhibitor NC or si-LINC00707 + miR-145 inhibitor were measured using an MTT assay. (D-E) Apoptotic cell rates of A549/DDP cells transfected with either si-LINC00707 + inhibitor NC or si-LINC00707 + miR-145 inhibitor were measured using flow cytometry. Protein expression levels of (F) Bcl-2 and Bax, and (G) MRP1 and P-gp in A549/DDP cells transfected with either si-LINC00707 + inhibitor NC or si-LINC00707 + miR-145 inhibitor were measured using western blotting. ***P<0.001 vs. si-LINC00707 + inhibitor NC. MRP1, multidrug resistance protein 1; P-gp, P-glycoprotein; IC50, half-maximal inhibitory concentration; LINC00707, long intergenic non-coding RNA 00707; miR, microRNA; si-LINC00707, LINC00707 siRNA; NC, negative control; A549/DDP cells, DDP-resistant A549 cells; RT-qPCR, reverse transcription-quantitative PCR.