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. 2019 Nov 13;10:1112. doi: 10.3389/fgene.2019.01112

Figure 4.

Figure 4

miR-216a regulates bovine primary muscle cells differentiation. (A) RT-qPCR was used to detect the messenger RNA (mRNA) expression of myogenic marker genes MyoD1, MyoG, and MyHC after transfection of miR-216a mimic. (B) Western blot was used to detect the protein expression level of MyoD, MyoG, and MyHC after transfection miR-216a mimic. (C) Protein quantitative analysis of MYHC, MYOG, and MYOD for (B). (D) The myotube formation of bovine primary muscle cells was measured by immunofluorescence assay under the 200 times field of microscope after transfection miR-216a mimic. (E) After loss of miR-216a, the mRNA expression of myogenic marker genes MyoD1, MyoG, and MyHC were detected by RT-qPCR. (F) After loss of miR-216a, the protein level of MyoD, MyoG, and MyHC were detected by western blot. (G) Protein quantitative analysis of MYHC, MYOG, and MYOD for (F). (H) After loss of miR-216a, the myotube formation of bovine primary muscle cells was measured by immunofluorescence assay under the 200 times field of microscope. Data are presented as the mean ± SEM; n = 3; *P < 0.05 and **P < 0.01.