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. 2019 Nov 11;25:8499–8508. doi: 10.12659/MSM.916458

Figure 6.

Figure 6

MAP2K4 is a target of miR-27a-3p and MAP2K4 expression in kainic acid-treated neurons. (A) Interaction between miR-27a-3p and 3′UTR of MAP2K4 was predicted using microRNA target site prediction software; (B) Luciferase activity of a reporter containing a wild-type MAP2K4 3′UTR or a mutant MAP2K4 3′ UTR are presented. “MAP2K4-MUT” indicates the MAP2K4 3′ UTR with a mutation in the miR-27a-3p binding site. UTR, untranslated region. (C, D) Neurons were treated with (KA) or without (Control) 50 μm kainic acid for 24 h, then the mRNA and protein expression of MAP2K4 in neurons was detected using qRT-PCR and Western blotting, respectively. Data are displayed as mean ±SD. ** p<0.01 vs. mimic control; ## p<0.01 vs. control group.