Figure 7.

Effect of miR-27a-3p inhibitor on the expression of MAP2K4 in neurons. (A) After transfection with inhibitor control or miR-27a-3p inhibitor for 48 h, the relative miR-27a-3p expression in neurons was detected using qRT-PCR. (B) After transfection with control-siRNA or MAP2K4-siRNA for 48 h, the mRNA expression of MAP2K4 in neurons was detected using qRT-PCR. (C, D) After transfection with inhibitor control, miR-27a-3p inhibitor, or miR-27a-3p inhibitor+MAP2K4-siRNA for 48 h, the mRNA and protein expression of MAP2K4 in neurons was detected using qRT-PCR and Western blotting, respectively. All data are presented as the mean ±SD of 3 independent experiments. ** p<0.01 vs. control group; ^## p<0.01 vs. inhibitor group.