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. Author manuscript; available in PMC: 2019 Nov 20.
Published in final edited form as: Methods Mol Biol. 2016;1454:169–192. doi: 10.1007/978-1-4939-3789-9_11

Fig. 4.

Fig. 4

Tuning the power of 405-nm laser line to control the activation efficiency of dyes. When seeing the reduction of number of molecules being localized through the imaging process, one should increase of the power of 405-nm laser to maintain an efficient imaging acquisition, minimizing the required number of frames for reconstructing the whole image. Ideally, number of molecule localization should be a constant during the acquisition