. 2019 Oct 7;8(11):1455–1467. doi: 10.1530/EC-19-0359
This work is licensed under a Table 2.
The independent and combined effects of sex and temperature on femur in mice housed at 22°C and 32°C.
| Variable | Female | Male | Sex | Temperature | Sex × temperature | ||
|---|---|---|---|---|---|---|---|
| Study completion (18 weeks old) | Study completion (16 weeks old) | P value | P value | P value | |||
| 22°C | 32°C | 22°C | 32°C | ||||
| Total femur | |||||||
| Length (mm) | 14.9 ± 0.1 | 14.8 ± 0.2 | 15.1 ± 0.1 | 15.1 ± 0.1 | 0.048 | NS | NS |
| Bone volume (mm3) | 16.6 ± 0.5 | 17.6 ± 0.6 | 18.5 ± 0.3 | 19.6 ± 0.2 | 0.000 | 0.055 | NS |
| Femur diaphysis | |||||||
| Cortical thickness (µm) | 192 ± 3 | 202 ± 4 | 189 ± 1 | 194 ± 1 | NS | 0.002 | NS |
| Distal femur metaphysis | |||||||
| Cancellous bone volume fraction (%) | 3.5 ± 0.4 | 9.9 ± 0.6 | 9.7 ± 0.6 | 15.4 ± 0.8 | 0.000 | 0.000 | NS |
| Trabecular number (1/mm) | 3.6 ± 0.1 | 4.7 ± 0.1 | 4.9 ± 0.1 | 5.8 ± 0.1 | 0.000 | 0.000 | NS |
| Trabecular thickness (µm) | 38 ± 1 | 45 ± 1 | 43 ± 1 | 44 ± 1 | NS | 0.007 | NS |
| Adipocyte area (%) | 4.4 ± 1.0 | 10.7 ± 1.4a | 0.3 ± 0.1 | 0.8 ± 0.3 | – | – | 0.005 |
| Adipocyte density (#/mm2) | 101 ± 24 | 196 ± 22a | 12 ± 2 | 16 ± 4 | – | – | 0.02 |
| Adipocyte size (µm2) | 472 ± 39 | 552 ± 51 | 249 ± 24 | 355 ± 66 | 0.000 | 0.091 | NS |
| Mineralizing perimeter/bone perimeter (%) | 24.4 ± 2.9 | 40.8 ± 1.8 | 15.8 ± 1.5 | 30.0 ± 1.4 | 0.000 | 0.000 | NS |
| Mineral apposition rate (µm/day) | 2.2 ± 0.1 | 2.0 ± 0.0 | 1.6 ± 0.2 | 1.7 ± 0.1 | 0.004 | NS | NS |
| Bone formation rate/bone perimeter (µm2/µm/year) | 190 ± 25 | 301 ± 17 | 92 ± 12 | 180 ± 13 | 0.000 | 0.000 | NS |
| Osteoblast perimeter/bone perimeter (%) | 17.7 ± 1.8 | 33.6 ± 2.0 | 15.8 ± 1.9 | 30.4 ± 1.6 | NS | 0.000 | NS |
| Osteoclast perimeter/bone perimeter (%) | 27.1 ± 3.0 | 13.1 ± 1.7a | 6.7 ± 1.6 | 4.7 ± 1.0 | – | – | 0.009 |
aDifferent from females at 22°C, P < 0.05. bDifferent from males at 22°C, P < 0.05.