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. 2019 Oct 9;18(6):5930–5940. doi: 10.3892/ol.2019.10972

Figure 2.

Figure 2.

Upregulation of JMJD3 promotes EMT in glioma cells. (A) Microscopy images of morphological alterations of U-251MG and U-87MG ATCC cells following JMJD3 overexpression. Scale bar, 100 µm. (B) mRNA expression levels of JMJD3 and EMT-associated genes (E-cadherin, N-cadherin and fibronectin) in U-251MG cells in which JMJD3 was overexpressed or depleted. (C) Western blot analysis examining the expression levels of EMT-associated proteins (E-cadherin and N-cadherin) in U-251MG cells in which JMJD3 was overexpressed or depleted. (D) Relative protein expression levels of E-cadherin and N-cadherin was determined in U-251MG cells. β-actin was used as an endogenous control. *P<0.05, **P<0.01. ATCC, American Type Culture Collection; EMT, epithelial-mesenchymal transition; JMJD3, Jumonji domain-containing protein 3; shRNA, short hairpin RNA.