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Primer sequences used to clone the sgRNAs targeting long non-coding RNA MEG3.
| Guide | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
| 5′ sgRNA_MEG3#1 | CACCGTTCGGGTATACGGCAGTCAC | AAACGTGACTGCCGTATACCCGAAC |
| 5′ sgRNA_MEG3#2 | CACCGCACTATTAACGCGATTCTAG | AAACCTAGAATCGCGTTAATAGTGC |
| 3′ sgRNA_MEG3#3 | CACCGTGAACTCTTGTCGGCCAGCG | AAACCGCTGGCCGACAAGAGTTCAC |
| 3′ sgRNA_MEG3#4 | CACCGAAGTGGGCCGAGTCTAAGGT | AAACACCTTAGACTCGGCCCACTTC |
MEG3, maternally expressed gene 3; sgRNA, single guide RNA.
