Propofol reduces miR-10b expression and increases HOXD10 levels in bladder cancer cells. (A) The relative expression levels of miR-10b in bladder cancer T24 cells following 48-h 10 µg/ml propofol treatment were detected by reverse transcription-quantitative PCR. Propofol treatment significantly reduced miR-10b expression levels in T24 cells. U6 was used as an internal control. Treatment of T24 cells with 10 µg/ml propofol significantly increased the (B) mRNA and (C) protein expression of HOXD10. GAPDH was used as an internal control. *P<0.05. Propofol, 2,6-diisopropylphenol; HOXD10, homeobox D10; U6, U6 small nuclear RNA; miR, microRNA.