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. 2019 Oct 8;18(6):6228–6236. doi: 10.3892/ol.2019.10968

Figure 3.

Figure 3.

HOXD10 is a direct target of miR-10b in bladder cancer cells. (A) Luciferase reporter constructs WT HOXD10 3′-UTR and MUT HOXD10 3′-UTR were inserted into a psiCHECK2 vector. Red indicates the miR-10b binding site. (B) miR-10b mimic significantly upregulated the expression levels of miR-10b in T24 cells compared with mimic-NC. (C) The relative luciferase activity of psiCHECK2-HOXD10-WT or MUT plasmid co-transfected with miR-10b mimic or mimic-NC in T24 cells. (D) Reverse transcription-quantitative PCR and (E) western blot assays demonstrated that the mRNA and protein levels of HOXD10 were reduced in T24 cells transfected with miR-10b mimic. GAPDH was used as a loading control. *P<0.05. miR, microRNA; HOXD10, homeobox D10; WT, wild type; MUT, mutant; UTR, untranslated region; NC, negative control.