Extended Data Fig. 4 |. Expression values and validation of top DEGs.

a, mean expression values of genes across the nuclei isolated from each individual. Each point represents one individual. DEGs were classified into low, mid, or highly expressed based on their median expression level across the cells of the corresponding cell-type. Groups were defined based on k-means clustering (k=3). Top 3 genes for each group and cell-types are shown. For Opc and Mic only 1 and 2 genes were classified within the high expression group. Box Plots are centered around the median, with interquartile ranges defining the box. b, RNA in-situ hybridization (RNAscope) with probes detecting excitatory neuron marker SLC17A7 (red) and NTNG1 (blue) in the grey matter of Brodmann area 10 (BA10) of a no-pathology and a pathology individual (left). The tissue was counterstained with hematoxylin. Right, quantification of RNA in situ hybridization on BA10 tissue sections (mean ± s.e.m.; *, P-Value < 0.05, Student’s two-tailed t-test, exact P value = 0.047; n=4 no-pathology and n=4 pathology individuals; n= 5, 6 images per individual).