Table 1.
Primers, accession numbers, and sequences used in real-time RT-PCR for all gene expression studies.
| Primer | Accession # | Oligo | Sequence |
|---|---|---|---|
| Gapdh1 | NM_017008 | Forward | 5’-ATGACTCTACCCACGGCAAG-3’ |
| Reverse | 5’-AGCATCACCCCATTTGATGT-3’ | ||
| IκBα2 | NM_080899 | Forward | 5’-CTGTTGAAGTGTGGGGCTGA-3’ |
| Reverse | 5’-AGGGCAACTCATCTTCCGTG-3’ | ||
| IL-13 | NM_031512 | Forward | 5’-AGGACCCAAGCACCTTCTTT-3’ |
| Reverse | 5’-AGACAGCACGAGGCATTTTT-3’ | ||
| IL-64 | NM_012589 | Forward | 5’-TAGTCCTTCCTACCCCAACTTCC-3’ |
| Reverse | 5’-TTGGTCCTTAGCCACTCCTTC-3’ | ||
| TNFα5 | NM_012675 | Forward | 5’-GGGGCCACCACGCTCTTCTG-3’ |
| Reverse | 5’-CGACGTGGGCTACGGGTTG-3’ |
1
Glyceraldehyde 3-phosphate dehydrogenase;
2
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha;
3
Interleukin-1 beta (β);
4
Interleukin-6;
5
Tumor necrosis factor alpha (α).