Figure 2.

GHRH antagonist suppresses the activation of JAK2, STAT3, ERK1/2, and induces the activation of AMPK in BPAECs.

Western Blot analysis of (a) Phosphorylated JAK2 (pJAK2) and JAK2, (b) Phosphorylated STAT3 (pSTAT3) and STAT3, (c) Phosphorylated ERK1/2 (pERK1/2) and ERK1/2, (d) Phosphorylated AMPK and AMPK after treatment of BPAEC with either vehicle (0.1% DMSO) or GHRH antagonist MIA 602 (1μM) for 24, 48 and 72 hours. The blots shown are representative of 3 independent experiments. The signal intensity of the protein bands was analyzed by densitometry. Protein levels of pJAK2, pSTAT3, pERK1/2 and pAMPK were normalized to JAK2, STAT3, ERK1/2 and AMPK respectively. *P < .05, **P < .01, ***P < .001 vs vehicle. Means ± SEM.