Figure 3.

Effects of GHRH in the P53, pJAK2, pSTAT3, pERK1/2 expression levels of BPAEC.

Western Blot analysis of (a) P53 and β-actin, (b) Phosphorylated JAK2 (pJAK2) and JAK2, (c) Phosphorylated STAT3 (pSTAT3) and STAT3, (d) Phosphorylated ERK1/2 (pERK1/2) and ERK1/2, after treatment of BPAEC with either vehicle (0.1% DMSO) or GHRH (1μM) for 24, 48 and 72 hours. The blots shown are representative of 3 independent experiments. The signal intensity of the protein bands was analyzed by densitometry. Protein levels of P53, pJAK2, pSTAT3, and pERK1/2 were normalized to β-actin, JAK2, STAT3 and ERK1/2 respectively. *P < .05, **P < .01 vs. vehicle. Means ± SEM.