Figure 5.

Effects of GHRH agonist MR 409 in the expression levels of pJAK2, pSTAT3, pERK1/2 in BPAEC.

Western Blot analysis of (a) Phosphorylated JAK2 (pJAK2) and JAK2, (b) phosphorylated STAT3 (pSTAT3) and STAT3, (c) Phosphorylated ERK1/2 (pERK1/2) and ERK1/2 after treatment of BPAEC with either vehicle (0.1% DMSO) or the GHRH agonist MR 409 (1μM) for 24, 48 and 72 hours. The blots shown are representative of 3 independent experiments. The signal intensity of the protein bands was analyzed by densitometry. Protein levels of pJAK2, pSTAT3, and pERK1/2 were normalized to JAK2, STAT3 and ERK1/2 respectively. *P < .05, **P < .01 vs vehicle. Means ± SEM.