Figure 2.

Influence of oral B₁₂ supplement on the fecal microbiome. Beta diversity analysis of (a) phylum and (b) genus level (n = 12 mice/time point) at baseline and following 16-day B₁₂ supplementation. *p < 0.05. (c) Significant reduction in the relative abundance of Bacteroides following B₁₂ supplementation (paired two-tailed t-test). (d) Cecal short-chain fatty acids in animals administered H₂O (Control) and experimental colitis (DSS) with and without B₁₂. (Control n = 15, B₁₂ n = 15, DSS n = 20, DSS/B₁₂ n = 18; paired two-tailed t-test (c) and ANOVA with Tukey’s post-test (d).