Fig. 1.

A comparison of conjugation efficiency using either the ΔCLAU_0514 strain of C. autoethanogenum DSM 10061 (IIG deficient) or the wild type strain as the recipient. sExpress was used as a conjugal donor in either case with the only difference in conjugation protocol being the choice of recipient strain. Error bars show the standard deviation. An unpaired two-tailed T-test was performed with Welch's correction to measure the significance of the difference between the two recipient strains giving a P value of <0.0001, n = 4. Four different recipient cultures were used for each condition with optical densities ranging from 0.1 to 0.2. Colony forming units (CFU) ml⁻¹ was calculated as the number of colony forming units per ml of recipient culture using 200 μl of conjugal donor culture per conjugation.