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. 2019 Oct 9;127(10):107004. doi: 10.1289/EHP4860

Figure 1.

Figure 1A is a conceptual diagram of an incubation chamber at 37°C. Following parts are labeled: oxygenator on the left (95 percent air, 5 percent C O sub 2); oxygenator on the right (95 percent N sub 2, 5 percent C O sub 2); pump on the left (12 milliliters per minute); and pump on the right (4 milliliters per minute). Figure 1B displays the photographs of the perfusion chamber containing the placental tissue from maternal and fetal side. Figure 1C is a conceptual diagram of the cannulation of the maternal and the fetal side with the following labelings: maternal reservoir; maternal perfusates; ZEN; pump (MA cannula and MV cannula); fetal reservoir; fetal perfusates; pump (FV cannula and FA cannula); placental villus; IVS; placental tissue; umbilical cord; fetal plasma; U V and UA.

Ex vivo dually perfused human placenta perfusion model. (A) Scheme of the perfusion system. (B) Photograph showing cannulation from the maternal and fetal side, respectively. (C) Scheme with details of maternal and fetal side cannulation of an intact cotyledon and sampling sites (magenta). Fetal plasma is isolated from the umbilical vein blood before perfusion. ZEN is introduced to the maternal reservoir at the start of perfusion. Maternal and fetal perfusates are sampled from the corresponding reservoirs at different time points during perfusion. Placental tissue (black quadrant) is taken from the intervillous region of a perfused cotyledon at the end of perfusion. Note: BT, bubble trap; FA, fetal artery; FV, fetal vein; IVS, intervillous space (maternal blood space); MA, maternal artery; MV, maternal vein; UA, umbilical artery; UV, umbilical vein.