Table 1.

Chromatographic and mass spectrometric parameters of the assessed analytes.

Analyte	Retention time (min)	Parent ion	Ion species	Quantifier ion	Qualifier ion	Ionization mode	Placental tissue (ng/g)		Fetal plasma ($\mathrm{\mu }\mathrm{g}/\mathrm{L}$)		Perfusion medium ($\mathrm{\mu }\mathrm{g}/\mathrm{L}$)
							LOD	LOQ	LOD	LOQ	LOD	LOQ
$\mathrm{\beta }\text{-ZEL-}14\text{-GlcA}$	3.82–4.03a	495	${\left[\mathrm{M}\text{-}\mathrm{H}\right]}^{-}$	319.3	175	Negative	2	6	5	15	1	1.5
$\mathrm{\alpha }\text{-ZEL-}14\text{-GlcA}$	4.10–4.35a	495	${\left[\mathrm{M}\text{-}\mathrm{H}\right]}^{-}$	319.3	175	Negative	6	6	5	5	0.5	1.5
ZEN-14-GlcA	4.39–4.64a	493.2	${\left[\mathrm{M}\text{-}\mathrm{H}\right]}^{-}$	317.2	175.1	Negative	6	20	5	15	1.5	5
ZEN-14-Sulf	5.36–5.57a	397.1	${\left[\mathrm{M}\text{-}\mathrm{H}\right]}^{-}$	317.2	175.1	Negative	2	6	0.15	0.5	0.15	0.5
$\mathrm{\beta }\text{-ZAL}$	8.08	321.2	${\left[\mathrm{M}\text{-}\mathrm{H}\right]}^{-}$	277.2	303.2	Negative	2	2	0.15	0.5	0.5	1.5
$\mathrm{\beta }\text{-ZEL}$	8.32	319.2	${\left[\mathrm{M}\text{-}\mathrm{H}\right]}^{-}$	275.2	160.1	Negative	2	2	0.5	1.5	0.5	1.5
$\mathrm{\alpha }\text{-ZAL}$	9.40	321.2	${\left[\mathrm{M}\text{-}\mathrm{H}\right]}^{-}$	277.2	303.2	Negative	2	2	0.15	0.5	0.5	0.5
$\mathrm{\alpha }\text{-ZEL}$	9.74	319.2	${\left[\mathrm{M}\text{-}\mathrm{H}\right]}^{-}$	275.2	160.1	Negative	2	6	0.5	1.5	0.5	1.5
ZAN	12.00	319.2	${\left[\mathrm{M}\text{-}\mathrm{H}\right]}^{-}$	275.2	205.2	Negative	2	2	0.15	0.5	0.15	0.5
ZEN	12.18	317	${\left[\mathrm{M}\text{-}\mathrm{H}\right]}^{-}$	175.0	131.0	Negative	0.6	2	0.05	0.15	0.05	0.15
${}_{}{}^{13}\mathrm{C}_{18}^{}\text{-ZEN}$ (IS)	12.18	335	${\left[\mathrm{M}\text{-}\mathrm{H}\right]}^{-}$	185.0	—	Negative

Note: —, indicate data not available, Multiple reaction monitoring (MRM) transitions, retention times and limit of detections (LOD), limit of quantifications (LOQ) of tested matrices. Zearalenone (ZEN), ZEN-14-glucuronide (ZEN-14-GlcA), ZEN-14-sulfate (ZEN-14-Sulf), $\mathrm{\alpha }\text{-}/\mathrm{\beta }\text{-zearalenol}$ ($\mathrm{\alpha }\text{-}/\mathrm{\beta }\text{-ZEL}$); $\mathrm{\alpha }\text{-}/\mathrm{\beta }\text{-ZEL -}14\text{-glucuronide}$ ($\mathrm{\alpha }\text{-}/\mathrm{\beta }\text{-ZEL-GlcA}$), $\mathrm{\alpha }\text{-}/\mathrm{\beta }\text{-zearalanol}$ ($\mathrm{\alpha }\text{-}/\mathrm{\beta }\text{-ZAL}$), zearalanone (ZAN); IS, Internal Standard.

^aMinor deviations in different matrices.