Table 1. Primers used in this study.
| Gene/CRISPR-Cas system type | Primer | Sequence | Purpose |
|---|---|---|---|
| cas1 | FOR Cas1 K2 | GCTGTTTGTCAAAGTTACCCGCGAACTC | For Cas1 gene |
| cas1 | REV Cas1 K2 | GGTTTTGATCGCCTCATGAGTCACAGTTG | For Cas1 gene |
| cse1 | FOR Cse1 K2 | CAGTTTAACCGATATTTTCAGCCAGCCGG | For Cse1 gene |
| cse1 | REV Cse1 K2 | CATCAGTTAATTGCTGCTGTTGCTGACTTTCG | For Cse1 gene |
| cas3 | FOR Cas3 K2 | GGGTTTCGCTACAAAATCAACATGCCATCG | For Cas3 gene |
| cas3 | REV Cas3 K2 | CACGAGTTTTTTACGCTCATCAAACCAGAGC | For Cas3 gene |
| I-E CRISPR1 | F-trp 1 |
CAGTTCCTGCAACCTGGCCT | For intact CRISPR1-Cas systems |
| I-E CRISPR1 | R-Cas2 2 |
CTGGCAGCAGGTGATACAGC | |
| I-E CRISPR1 | F-iap 3 |
CTGGCATAACGCCACCGG | For incomplete CRISPR1-Cas systems |
| I-E CRISPR1 | R-cysH 4 |
GAGACCCGGTTCTTCGGGC | |
| I-E* CRISPR2 | FU-Cas3 5 |
GTAGCGAAACCCTGATCAAGCG | For intact CRISPR-Cas systems IE*-CRISPR2 |
| I-E* CRISPR2 | R-L2 6 |
GCGCTACGTTCTGGGGATG | |
| I-E* CRISPR2 | R-L2 6 |
GCGCTACGTTCTGGGGATG | For incomplete CRISPR-Cas systems IE*-CRISPR2 |
| I E* CRISPR2 | F-HP2 7 |
CGTCGCAAAACTCGACCAGA | |
| I-E* CRISPR3 | F-HP1 8 |
GACGCTGGTGCGATTCTTGAG | For intact CRISPR-Cas systems IE*-CRISPR3 |
| I-E* CRISPR3 | RU-Cas2 9 |
CGCAGTATTCCTCAACCGCCT | |
| For CRISPR deletion | Down-70 | GCCGCGATGGCATGTTGATTTTGTAGCGAAACCCTGATCAAGCGCCTCATCATATGAATATCCTCCTTAG | For I-E* CRISPR2 deletion |
| For CRISPR deletion | Up-70 | CGCGCTACGTTCTGGGGATGACAAAAGCGTTTTACCCCCGGCTGCGGGCCGTGCAGGCTGGAGCTGCTTC | For I-E* CRISPR2 deletion |