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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Exp Eye Res. 2019 Aug 31;188:107787. doi: 10.1016/j.exer.2019.107787

Fig. 6.

Fig. 6.

Quantitative real-time PCR of lens-enriched genes in SRA01/04 and HEK293T cell lines. Several robustly expressed lens genes (CRYAB, DKK3, EPHA2, ETV5, ITGB1, SFRP1, VIM) were subjected to RT-qPCR and were found to be significantly enriched in SRA01/04 cells. The lens fiber cell gene PROX1 was also examined by RT-qPCR analysis and was found to be significantly downregulated in SRA01/04 compared with HEK293T. RT-qPCR analysis was performed in biological triplicates and technical triplicates, ΔΔCT method used to calculate fold change, and nested ANOVA was used to determine significance. Asterisks denote a statistically significant difference (p<0.05).