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. 2019 Nov 20;14(11):e0224314. doi: 10.1371/journal.pone.0224314

Fig 4. miR-200c regulates migration associated genes such as filamin A.

Fig 4

(a) A proteomic analysis of a TALENs knock-out (KO) of miR-200c in MCF7 cells revealed a set of proteins with differential expression, of which 50% are involved in migratory processes and are shown in the table. (RT qPCR showed that after adding 5 μg / ml doxycycline for 48 h the expression of FLNA mRNA (b) as well as filamin A protein(c) (normalized to tubulin) decreased significantly in MDA-MB 231 TRIPZ 200c cells. (d) Immunofluorescence staining of filamin A (green) and DAPI (blue) in MCF7 Ctrl and KO 200c showed significantly increased relative intensity of filamin A, in contrast to (e) the MDA-MB-231 cells which showed a strong decrease in filamin A intensity after induction of miR-200c (all N = 3; error bars indicate standard deviation SD; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≥ 0.0001).