Figure 1. Graphical representation of the experimental approach.

(A) The natural progress of streptococcal infection, with highlighted sampling times during the acute and resolution phases of infection. The Y axis indicates the percentage of surviving adults. (B) The approach used to isolate hemocytes, which are subsequently assayed for gene expression and enzymatic activities. Macrophages sorted from flies at the respective time points post-infection represent acute-phase macrophages (APMФs; 24 hpi) and resolution-phase macrophages (RPMФs; 120 hpi). Control flies were analyzed at the same time points after receiving injection of phosphate-buffered saline (PBS). hpi, hours post-infection; FACS, fluorescence-activated cell sorting; S.p., Streptococcus pneumoniae.