Key resources table.
| Reagent type (species) or resource |
Designation | Source reference |
Identifier | Additional information |
|---|---|---|---|---|
| Strain, strain background (Streptococcus pneumoniae) | EJ1 strain | Provided by David Schneider | Dilution 20,000 units | |
| Chemical compound, drug | TRIzol Reagent | Invitrogen | Cat# 15-596-018 | |
| Chemical compound, drug | Superscript III Reverse Transcriptase | Invitrogen | Cat# 18080044 | |
| Chemical compound, drug | 2x SYBR Master Mix | Top-Bio | Cat# T607 | |
| Chemical compound, drug | 2-NBDG | Thermo Fisher Scientific |
Cat# N13195 | |
| Chemical compound, drug | X-gal | Sigma | Cat# B4252 | |
| Commercial assay, kit | Glucose (GO) Assay Kit | Sigma | Cat# GAGO20-1KT | |
| Commercial assay, kit | Bicinchoninic Acid Assay Kit | Sigma | Cat# BCA1 | |
| Commercial assay, kit | Lactate Assay Kit | Sigma | Cat# MAK064 | |
| Commercial assay, kit | Lactate Dehydrogenase Activity Assay Kit | Sigma | Cat# MAK066 | |
| Commercial assay, kit | Phosphoglucose Isomerase Colorimetric Assay Kit | Sigma | Cat# MAK103 | |
| Genetic reagent (Drosophila
melanogaster) |
HmlG4G80: w*; HmlΔ-Gal4*; P{tubPGal80ts}* | Cross made in our laboratory by Tomas Dolezal | ||
| Genetic reagent (D. melanogaster) | Hml > GFP: w; HmlΔ-Gal4 UAS-eGFP | Provided by Bruno Lemaitre | ||
| Genetic reagent (D. melanogaster) |
Hif1α[RNAi]: P{KK110834}VIE-260B | Vienna Drosophila Resource Center | VDRC: v106504 | FBst0478328 |
| Genetic reagent (D. melanogaster) | TRiP control: y(1) v(1); P{y[+t7.7]=CaryP}attP2 | Bloomington Drosophila Stock Center |
BDSC: 36303 | FBst0036303 |
| Genetic reagent (D. melanogaster) | KK control: y, w[1118];P{attP,y[+],w[3`]} | Bloomington Drosophila Stock Center | BDSC: 60100 | FBst0060100 |
| Genetic reagent (D. melanogaster)
|
Ldh[RNAi]: y(1) v(1); P{y[+t7.7] v[+t1.8]=TRiP.HMS00039}attP2 | Bloomington Drosophila Stock Center |
BDSC: 33640 | FBst0033640 |
| Genetic reagent (D. melanogaster) | HRE-LacZ: HRE-HRE-CRE-LacZ | Provided by Pablo Wappner (Lavista-Llanos et al., 2002) | ||
| Genetic reagent (D. melanogaster) | Ldh-mCherry | Provided by Jason Tennessen | ||
| Genetic reagent (D. melanogaster) | w: w1118 | Genetic background based on CantonS | ||
| Sequence-based reagent | Cis forward: 5′TTCGATTGACTCCAGCCTGG3′ | KRD | CG14740 | FBgn0037988 |
| Sequence- based reagent |
Cis reverse: 5′AGCCGGGAACCACCTGTCC3′ | KRD | CG14740 | FBgn0037988 |
| Sequence-based reagent | Ldh forward: 5′CAGAGAAGTGGAACGAGCTG3′ |
KRD | CG10160 | FBgn0001258 |
| Sequence- based reagent |
Ldh reverse: 5′CATGTTCGCCCAAAACGGAG3′ |
KRD | CG10160 | FBgn0001258 |
| Sequence-based reagent | Eno forward: 5′CAACATCCAGTCCAACAAGG3′ | KRD | CG17654 | FBgn0000579 |
| Sequence-based reagent | Eno reverse: 5′GTTCTTGAAGTCCAGATCGT3′ | KRD | CG17654 | FBgn0000579 |
| Sequence-based reagent | Gapdh1 forward: 5′TTG TGG ATC TTA CCG TCC GC3′ |
KRD | CG12055 | FBgn0001091 |
| Sequence-based reagent | Gapdh1 reverse: 5′CTCGAACACAGACGAATGGG3′ | KRD | CG12055 | FBgn0001091 |
| Sequence-based reagent | HexA forward: 5′ATATCGGGCATGTATATGGG3′ | KRD | CG3001 | FBgn0001186 |
| Sequence-based reagent | HexA reverse: 5′CAATTTCGCTCACATACTTGG3′ |
KRD | CG3001 | FBgn0001186 |
| Sequence-based reagent | Pfk forward: 5′AGCTCACATTTCCAAACATCG3′ |
KRD | CG4001 | FBgn0003071 |
| Sequence-based reagent | Pfk reverse: 5′TTTGATCACCAGAATCACTGC3′ | KRD | CG4001 | FBgn0003071 |
| Sequence-based reagent | Pgi forward: 5′ACTGTCAATCTGTCTGTCCA3′ | KRD | CG8251 | FBgn0003074 |
| Sequence-based reagent | Pgi reverse: 5′GATAACAGGAGCATTCTTCTCG3′ | KRD | CG8251 | FBgn0003074 |
| Sequence-based reagent | Rp49 forward: 5′AAGCTGTCGCACAAATGGCG3′ | KRD | CG7939 | FBgn0002626 |
| Sequence-based reagent | Rp49 reverse: 5′GCACGTTGTGCACCAGGAAC3′ | KRD | CG7939 | FBgn0002626 |
| Sequence-based reagent | Hif1α forward: 5′CCAAAGGAGAAAAGAAGGAAC3′ | KRD | CG45051 | FBgn0266411 |
| Sequence-based reagent | Hif1α reverse: 5′GAATCTTGAGGAAAGCGATG3′ | KRD | CG45051 | FBgn0266411 |
| Sequence-based reagent | CG10219 forward: 5′GAGATCTCCGTGAGTGCGC3′ | KRD | CG10219 | FBgn0039112 |
| Sequence-based reagent | CG10219 reverse: 5′CTCCACGCCCCAATGGG3′ | KRD | CG10219 | FBgn0039112 |
| Sequence-based reagent | Scsα1 forward: 5′TCACAAGCGCGGCAAGATC3′ | KRD | CG1065 | FBgn0004888 |
| Sequence-based reagent | Scsα1 reverse: 5′TTGATGCCCGAATTGTACTCG3′ | KRD | CG1065 | FBgn0004888 |
| Sequence-based reagent | Tpi forward: 5′AGATCAAGGACTGGAAGAACG3′ | KRD | CG2171 | FBgn0086355 |
| Sequence-based reagent | Tpi reverse: 5′ACCTCCTTGGAGATGTTGTC3′ | KRD | CG2171 | FBgn0086355 |
| Software, algorithm | Graphpad Prism | https://www.graphpad.com/ | Graphpad Prism | RRID:SCR_002798 |
| Software, algorithm | Microsoft Excel | https://www.microsoft.com/ | Microsoft Excel | |
| Software, algorithm | Fiji | ImageJ - https://fiji.sc | ImageJ | RRID:SCR_002285 |
| Other | S3e Cell Sorter | BioRad -http://www.bio-rad.com/ | BioRad | |
| Other | Olympus FluoView 1000 | Olympus -https://www.olympus-global.com/ | Olympus | RRID:SCR_017015
RRID:SCR_014215 |
| Other | Olympus SZX12 | Olympus -https://www.olympus-global.com/ | Olympus | |
| Other | Olympus IX71 | Olympus - https://www.olympus-global.com/ |
Olympus |