Key resources table.
Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (Streptococcus agalactiae) |
BM110 | Musser et al., 1989 | N/A | Isolated from patient |
Strain, strain background (Streptococcus agalactiae) | BM110∆hvgA | Tazi et al., 2010 | CCH395 | |
Strain, strain background (Streptococcus agalactiae) | BM110∆srr2 | This study | CCH2040 | Constructed by in-frame deletion (see Materials and methods, section Bacterial strains) |
Strain, strain background (Streptococcus agalactiae) | BM110 BTWT∆srr2 | This study | CCH2041 | (see Materials and methods, section Bacterial strains) |
Strain, strain background (Streptococcus agalactiae) | NEM316 | Glaser et al., 2002 | N/A | Isolated from patient |
Cell line (Homo sapiens) | Caco-2/TC7 Colonic epithelial cell | Chantret et al., 1994 | RRID:CVCL_0233 | |
Cell line (Homo sapiens) | Raji B lymphocytes | ATCC | CCL-86; RRID:CVCL_0511 | |
Antibody | anti-group B Streptococcus (Rabbit polyclonal) | ThermoFisher | PA1-7250; RRID:AB_561572 | IF (1:100) |
Antibody | anti-group B Streptococcus (Mouse serum) | This study | N/A | Homemade (see Materials and methods, section Reagents) IF (1:100) |
Antibody | anti-human glycoprotein 2 (Rabbit polyclonal) | Abcam | ab129414; RRID:AB_11155051 | IF (1:100) |
Antibody | anti-human glycoprotein 2 (Rabbit polyclonal) | Abcam | ab105503, RRID:AB_10859272 | WB (1:300) |
Antibody | anti-mouse CD11c- Alexa Fluor 488 (Armenian hamster monoclonal) | Biolegend | 117311; RRID:AB_389306 | IF (1:100) |
Antibody | anti-mouse CD11c- Alexa Fluor 647 (Armenian hamster monoclonal) | Biolegend | 117312; RRID:AB_389328 | IF (1:100) |
Antibody | anti-mouse CD11c- Brilliant Violet 785 (Armenian hamster monoclonal) | Biolegend | 117335, RRID:AB_11219204 | FACS (5 µL per test) |
Antibody | anti-mouse F4/80- PE/Cy5 (Rat monoclonal) | Biolegend | 123111, RRID:AB_893494 | FACS (5 µL per test) |
Antibody | anti-mouse CD19- Brilliant Violet 711 (Rat monoclonal) | Biolegend | 115555, RRID:AB_2565970 | FACS (5 µL per test) |
Antibody | anti-mouse CD45-APC (Rat monoclonal) | Biolegend | 103111, RRID:AB_312976 | FACS (5 µL per test) |
Antibody | anti-mouse I-A/I-E (anti-MHC-II)-FITC (Rat monoclonal) |
Biolegend | 107605, RRID:AB_313320 | FACS (5 µL per test) |
Chemical compound, drug |
UEA-I-Rhodamine | Vector Laboratories | RL-1062, RRID:AB_2336769 | IF (1:200) |
Chemical compound, drug | Estradiol | Sigma-Aldrich | E2758; CAS 50-28-2 |
|
Chemical compound, drug | Progesterone | Sigma-Aldrich | P8783; CAS 57-83-0 |
|
Software, algorithm | Kaluza | Beckman Coulter | RRID:SCR_016182 | |
Software, algorithm | Image J | http://imagej/net | RRID:SCR_003070 |