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. 2019 Nov 20;39(47):9306–9315. doi: 10.1523/JNEUROSCI.1566-19.2019

Figure 2.

Figure 2.

Tiam1 knockdown does not affect AMPAR- or NMDAR-mediated neurotransmission in CA1 pyramidal neurons. A, Hippocampal Tiam1 mRNA expression data from the Allen Mouse Brain Atlas. Scale bar, 200 μm. Dashed blue box shows enlarged CA1 region. B, Immunolabeling of Tiam1 in CA1 pyramidal neurons (top) and DG granule neurons (bottom) in hippocampal slices. SP, stratum pyramidale; SR, stratum radiatum; GL, granule layer; ML, molecular layer. C, Schematic representation of electrophysiological recording setup for CA1 pyramidal neurons. D, E, Scatterplots show eEPSC amplitudes for pairs of untransfected and transfected cells (open circles) with corresponding mean ± SEM (filled circles). Insets, Representative current traces from control and transfected (green) neurons with stimulation artifacts removed. Calibration: 20 pA for both AMPAR-eEPSC and NMDAR-eEPSC, 20 ms for AMPAR-eEPSC, 50 ms for NMDAR-eEPSC. Barplots show the average AMPAR-eEPSC and NMDAR-eEPSC amplitudes (±SEM) of CA1 pyramidal neurons expressing Tiam1 shRNA normalized to their respective control cell average eEPSC amplitudes. Tiam1 shRNA expression did not significantly affect AMPAR-eEPSC amplitude (n = 9 pairs) or NMDAR-eEPSC amplitude (n = 8 pairs) in CA1 pyramidal neurons. n.s., Not significant, Wilcoxon signed-rank test.