FIG 3.

Effect of IL-27p28 overexpression on T cell responses in the brain during toxoplasmic encephalitis. (A) Expression of CXCR3 and KLRG1 by LFA1^hi CD4⁺ and CD8⁺ T cells in brains and the relative percentages of the CXCR3⁻ KLRG1⁺ subset are compared between WT and IL-27p28 mice. (B) Class II and class I tetramers to detect T. gondii-specific CD4⁺ and CD8⁺ T cells in the brains of infected WT and IL-27p28 transgenic mice. (C and D) Expression of IFN-γ and T-bet in LFA1^hi CD4⁺ and CD8⁺ T cells (C) and their production of IL-10 and IL-17A (D). Representative flow plots are shown, while histograms represent the means (±SEM; n = 6 to 8) from 2 independent experiments.