TABLE 2.

Measured ELISA data for the recognition of antigens (rErpY-like protein and rLoa22) using sera of hosts (human, bovine, and canine) testing positive or negative for leptospirosis by MAT

Host and MAT serum result (no. of samples)	Recombinant protein	OD (means ± SD)	Cutoff (means + 2 SD)	Sensitivity (%)	Specificity (%)
Human
Positive (50)	rErpY-like	0.3587 ± 0.0230	_	100	_
	rLoa22	0.6339 ± 0.0360	_	100	_
Negative (20)	rErpY-like	0.2268 ± 0.0308	0.2883	_	100
	rLoa22	0.4478 ± 0.0212	0.4903	_	100
Bovine
Positive (50)	rErpY-like	0.3684 ± 0.0373	_	100	_
	rLoa22	0.6404 ± 0.0194	_	100	_
Negative (20)	rErpY-like	0.2431 ± 0.0236	0.2902	_	100
	rLoa22	0.4401 ± 0.0223	0.4846	_	100
Canine
Positive (18)	rErpY-like	0.2497 ± 0.0143	_	100	_
	rLoa22	0.4215 ± 0.0191	_	100	_
Negative (11)	rErpY-like	0.1291 ± 0.0198	0.1687	_	100
	rLoa22	0.2578 ± 0.0207	0.2992	_	100