FIG 4.

MI was detectd by CFU determination. All antisera were heated at 56°C for 30 min before use; fresh rabbit serum (RS) served as the source of complement, and heat-inactivated rabbit serum (HIRS) served as a control. Shown is growth of M. bovis in PPLO broth containing negative serum (A), anti-M. bovis serum (B), anti-PDHE2 serum (C), anti-PNP serum (D), anti-P81 serum (E), or anti-UgpB serum (F) at a dilution of 1:40 along with RS or HIRS (as the control group) at a dilution of 1:20. These data are presented as the means ± SD from three separate experiments.