TABLE 1.
Identification of the target proteins by LC-MS/MSa
| Spot no. | Protein name | Protein abbreviation | GenBank accession no. | MW (kDa) | Protein score |
|---|---|---|---|---|---|
| 1 | Pyruvate dehydrogenase E2 component | PDHE2 | AIA34105.1 | 8.59 | 811 |
| 2 | Membrane lipoprotein P81 | P81 | AIA34118.1 | 81.60 | 20076 |
| 3 | Glycerol ABC transporter, glycerol binding protein | UgpB | AIA34272.1 | 70.12 | 7213 |
| 4 | Pyruvate dehydrogenase E1 component subunit alpha | PDHA | AIA33689.1 | 41.33 | 13433 |
| 5 | Pyruvate dehydrogenase E1 component subunit beta | PDHB | AIA33690.1 | 36.18 | 13774 |
| 6 | Purine nucleoside phosphorylase | PNP | AIA34127.1 | 25.92 | 6237 |
| 7 | Phosphoketolase | PK | AIA33718.1 | 89.94 | 9651 |
| 8 | Hypothetical protein | AIA33828.1 | 69.82 | 9078 | |
| 9 | Lipoprotein | AIA34109.1 | 85.52 | 8954 |
a
The target protein bands were cut out after separation by SDS-PAGE and subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS). MW, molecular weight.