FIG 2.

Akt inhibitors imitate and a GSK3 inhibitor reverses the blunted CD40 upregulation in the presence of pLL, whereas mTORC1 inhibitors are without effect. GM-CSF–BMDCs were exposed to pLL, LPS, or both stimuli together for 18 h in the absence or presence of inhibitors, and the surface expression of CD40 was measured. The inhibitors used interfere with Akt activation (Akt inhibitor VIII, triciribine, and amlexanox) (a), GSK3 activity (SB216763) (b), and mTORC1 activity (rapamycin and torin1) (c). Graphs show means ± the SD of triplicate wells. In the presence of torin1, the difference between LPS only and LPS+pLL is P = 0.052. The results shown are representative of two (Akt inhibitor VIII and triciribine) or three (amlexanox, SB261763, and mTORC1 inhibitors) independent experiments.