FIG 3.

The changes induced by pLL in GM-CSF–BMDCs on CD86 and IL-10 expression are independent of alterations in Akt/GSK3 signaling. GM-CSF–BMDCs were exposed to pLL, LPS, or both stimuli together for 18 h in the absence or presence of inhibitors, and the cell surface expression of CD86 (a and b) and the levels of IL-10 (c and d) and IL-12p70 (e and f) in the supernatants were measured. The inhibitors used interfere with Akt activation (Akt inhibitor VIII, triciribine, and amlexanox) or GSK3 activity (SB216763). All data shown correspond to means ± the SD of triplicate wells. The results shown are representative of two (Akt inhibitor VIII and triciribine) or three (amlexanox and SB261763) independent experiments.