FIG 4.

Neither Akt/GSK3 phosphorylation nor CD40 upregulation is blunted by pLL in Flt3L-BMDCs. Flt3L-BMDCs were exposed to pLL, LPS, or both stimuli together. After 18 h, the cells were analyzed for cell surface expression of CD40 and CD86 (a and d) or for IL-10 and IL-12/23p40 levels in supernatants (e and f). Alternatively, after the times indicated, cell lysates were generated for Western blot analysis (b and c); the graphs show of the quotients of p-Akt or p-GSK3a/b values (normalized over loading controls) for cells treated with pLL over cells not exposed to pLL (means and ranges of two independent experiments). Data plotted in panels a, d, e, and f represent means ± the SD of triplicate wells and are representative of at least two independent experiments; significant enhancement by pLL of CD40 expression induced by 100 ng/ml LPS was not reproduced across experiments.