FIG 6.

The phenotypic effects of pLL on GM-CSF–BMDCs require functional class I PI3Ks. GM-CSF–BMDCs were exposed to pLL, LPS, or both stimuli together for 18 h in the absence or presence of the pan-PI3K inhibitor wortmannin (a to d) or the PI3K class I-specific inhibitor GDC-0941 (e to h). The cell surface expression of CD40 (a and e) and CD86 (b and f) and the levels of IL-10 (c and g) and IL-12p70 (d and h) in supernatants were measured. All values plotted correspond to means ± the SD of triplicate wells. The results shown are representative of at least three independent experiments.