FIG 8.

The diminished capacity to upregulate CD40 can be transmitted to cells not in contact with pLL. (a) GM-CSF–BMDCs were cultured for 18 h using permeable transwell inserts in the presence of pLL, LPS, or both stimuli; pLL when added was present in the lower compartment only, whereas LPS (when added) was present in both compartments, as depicted. (b) The cell surface expression of CD40 was then measured in cells from both compartments separately. This allowed us to assess the potential of cells in contact with pLL to condition cells not in contact with pLL via diffusible factors. The values plotted correspond to means ± the SD of triplicate wells. The results shown are representative of three independent experiments.