FIG 2.

E. chaffeensis infection and TRIM21 colocalization in the presence of OMP-1-specific huMAb. THP-1 cells were infected with E. chaffeensis in the presence of anti-OMP-1 huMAbs and fixed 30 min postinfection. Confocal immunofluorescence microscopy shows internalization of anti-OMP-1 EHRL-4-bound (green) E. chaffeensis (red) in THP-1 cells (A) and anti-OMP-1 EHRL-4-bound E. chaffeensis complexes (red) colocalizing with TRIM21 (green) intracellularly (B). Cells were visualized under 63× oil immersion lens. Bars, 10 μm.