FIG 3.

Role of TRIM21 in inhibition of E. chaffeensis infection by OMP-1 huMAb EHRL-4. THP-1 WT and TRIM21 KO cells were infected with E. chaffeensis opsonized with huMAbs (EHRL-2, -4, and -15), and ehrlichial infection was determined on day 3 postinfection by qPCR. (A) TRIM21 KO cells were generated using genome editing by CRISPR-Cas9, and the WT and KO cells were analyzed by Western blotting for expression of TRIM21 protein. (B) E. chaffeensis infection was determined 3 days postinfection by qPCR in WT and TRIM21 KO cells. Bar graphs represent means ± SEMs. *, P < 0.05 when comparing WT to WT; ^#, P < 0.05 when comparing KO to WT for each huMAb.