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. 2019 Nov 18;87(12):e00383-19. doi: 10.1128/IAI.00383-19

FIG 4.

FIG 4

OMP-1 huMAb EHRL-4-E. chaffeensis complex detection by TRIM21 initiates immune signaling. THP-1 was infected with E. chaffeensis in the presence or absence of EHRL-4, and samples were collected for confocal microscopy and examination of cytoplasmic and nuclear fractions at various time points postinfection. (A) Confocal images show TRIM21 (green) recruited to the E. chaffeensis-EHRL-4 complexes colocalizing with K63- and K48-linked Ub chains (red) at 30 min postinfection. Bars, 10 μm; ×63 magnification (oil immersion lens). The control (ctrl) and E. ch panels represent the merged images for TRIM21 and K63/48-linked Ub chains in uninfected and infected cells. Western blot analysis of nuclear (B) and cytoplasmic (C) fractions detecting activation/nuclear translocation of NF-κB and TRIM21. Bar graphs represent densitometric analysis of the Western blots with means ± SEMs. *, P < 0.05.