FIG 5.

OMP-1 huMAb EHRL-4-E. chaffeensis complex detection by TRIM21 mediates degradation and a proinflammatory response. THP-1 cells were infected with E. chaffeensis in the presence or absence of EHRL-4, and samples were collected for whole-cell lysates and evaluation of RNA expression at various time points postinfection. (A) Degradation of OMP-1 and EHRL-4 (IgG), with bar graphs representing densitometric analysis of the Western blots with means ± SEMs. *, P < 0.05. (B) mRNA expression levels of proinflammatory cytokines and chemokines in THP-1 cells by real-time reverse transcription-PCR. mRNA levels were normalized to GAPDH and compared with the level of uninfected cells. Bar graphs represent means ± SEMs. *, P < 0.05.