FIG 6.

OMP-1 huMAb EHRL-4 mediates intracellular degradation of E. chaffeensis by autophagy. THP-1 cells were infected with E. chaffeensis in the presence or absence of EHRL-4 and examined for autophagic flux and degradation of ehrlichiae. (A) Western blot analysis of 10 μg of the whole-cell extract showing autophagic flux, detecting accumulation and degradation of p62 and OMP-1 and increasing levels of LC3II when inoculated with EHRL-4-opsonized ehrlichiae. Bar graphs represent densitometric analysis of the Western blots with means ± SEMs. *, P < 0.05. (B) Transcript levels of dsb, demonstrating bacterial loads at various time points postinfection. Bar graphs represent means ± SEMs. *, P < 0.05.