FIG 7.

OMP-1 huMAb EHRL-4-opsonized E. chaffeensis engages TRIM21, which recruits autophagy regulators and effectors. THP-1 cells were infected with E. chaffeensis in the presence or absence of EHRL-4 and examined for interaction with autophagy regulator/effectors at 30 min postinfection. Confocal images showing colocalization of TRIM21 (red) and autophagy regulators ULK1 (green) and beclin 1 (green) (A), colocalization of TRIM21 (red) with sequestosome 1/p62 (green) and mammalian Atg8s (LC3, green) (B), and colocalization of E. chaffeensis (red) with LAMP2 (green), only in the presence of EHRL-4 (C). Bars, 10 μm; ×63 magnification (oil immersion lens).