FIG 3.

Gb3 and Gb4 distribution in Vero cells. Gb3 and Gb4 antibodies were applied independently (A and B) or simultaneously (C) to Vero cells before analysis by cytofluorometry. (A) Staining pattern for the combination of a rat antibody against Gb3 and an allophycocyanin (APC)-conjugated goat anti-rat IgG antibody (red line). (B) Staining pattern for the combination of a rabbit antibody against Gb4 and a phycoerythrin (PE)-conjugated donkey anti-rabbit IgG antibody (red line). Cells incubated with the secondary antibody alone (black lines in panels A and B) were processed as well. (C) The combined staining patterns for Gb3 and Gb4 are represented on a scatter plot of 10,000 individual cells. Cells exposed to secondary antibody alone were used to set background gates represented by the red lines.