FIG 1.

Strains 33277 and 381 differentially activate HEK cell TLR2-dependent, but not HEK cell TLR4-dependent, signaling. (A) HEK cells expressing TLR2 were stimulated with either 10⁵ or 10⁶ bacteria of both strains 33277 and 381. (B) HEK cells expressing TLR4 were stimulated with either 10⁵ or 10⁶ bacteria of both strains 33277 and 381. Fold change in NF-κB activation from bacterial stimulations is shown relative to the unstimulated control. Data are presented as means ± standard deviation (SD) from triplicate sample determinations and are representative of three independent experiments. Asterisks indicate statistical significance as determined by Student’s unpaired t test (**, P < 0.001; ***, P < 0.0001). ns, not statistically significant.