FIG 10.
Isogenic mutant strains of 33277 and 381 that are devoid of CTD proteins display increased immune-stimulatory activity. (A) HEK cells expressing TLR2 were stimulated with wild-type strains 33277 and 381 and the indicated isogenic mutant strains (105 bacteria). Fold change in NF-κB activation from bacterial stimulations is shown relative to the unstimulated control. (B) Differentiated THP-1 cells were stimulated with wild-type strains 33277 and 381 and the indicated isogenic mutant strains (105 bacteria). Fold change in IL-1β production from bacterial stimulations is shown relative to the unstimulated control. Data are presented as means ± SD from triplicate sample determinations and are representative of two independent experiments. Asterisks indicate statistical significance as determined by Student’s unpaired t test (*, P < 0.05; **, P < 0.001).