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ZnuD is required for growth in low Zn conditions. WT and ΔznuD strains were grown in LB (A) and LB + 40 μM TPEN or LB + 40 μM TPEN + 40 μM ZnCl2 (B), and OD600 was monitored over time. (C) WT and ΔznuD strains harboring empty pWH1266 expression vector or pWH1266 containing znuD were grown in LB + 20 μM TPEN, and OD600 was monitored over time. Data are shown in biological triplicate as mean ± SD and are representative of three independent experiments.
